MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
IKKbeta is a member of the IKK complex which is composed of IKKalpha, IKKbeta, IKKgamma and IKAP.
IkB Kinase (IKK) complex is a critical component in NFkB regulation.
In a majority of unstimulated cells, the NFkB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IkB proteins.
For NFkB to become activated, it must first disassociate from the inhibitor IkB.
This occurs via the serine phosphorylation of IkB on Ser32 and Ser36.
Post receptor stimulation, most notably by TNFalpha, NIK, a serine/threonine kinase, becomes activated and phosphorylates IKKalpha and IKKbeta.
Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1.
Once activated, IKKbeta autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response.
Additionally, IKKbeta activity can also be regulated by MEKK-1.
IKK then binds to and phosphorylates IkBalpha on the two serine residues.
This phosphorylation marks IkB for ubiquination and destruction by the proteosome.
This free the NFkB complex and allows it to translocates to the nucleus.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000 (1ug/ml)Immunohistochemistry (Paraffin): 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A431 cell lysate, HeLa cells
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human IKK beta. Does not cross react with IKK alpha or IKK gama. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa662-680 of human IKK beta, which differs from the corresponding murine sequence by one amino acid. Cellular Localization: Cytoplasm.
