MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications: Western blot: 1:500–1:1,000 Immunoprecipitation EIA Supershift assay: 0.5–1.0ml per assay.
Storage and Stability: Maintain frozen at-20ºC in undiluted aliquots for up to six months.
Avoid repeated freeze/thaw cycles.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified Ig
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity:Recognizes human cyclic AMP responsive element binding protein-1 (CREB-1, p43). By Western blot it shows a major band at 46kD when assayed against a lysate of 1x10e6 Raji B cells. Minor bands at 75kD and 43kD may be noted. Partial reactivity to CREM-1 protein. May react non-specifically with other proteins. Species Crossreactivity: Human, rat, mouse.
Isotype:IgG
Calc Applications Abbrev:FLISA IP WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:CREB-1 (p43) peptide corresponding to the N-terminal (aa 5–45) of human protein conjugated to KLH.