MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Recommended Dilution:Western Blot: detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:Jurkat lysate.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes the PRMT1 doublet, Mr ~42- 44kD. A non-specific protein was also detected, Mr ~120kD. Species Crossreactivity: Human and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa298-318 (KTGEEIFGTIGMRPNAKNNRD) of human protein arginine methyltransferase 1 (accession number CAA71765). The immunizing sequence is identical in mouse.
