MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: detects Stargazin in RIPA lysates from rat brain microsomal preparation.
Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Stargazin (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Immunoprecipitation: has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes rat Stargazin.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Rt
Immunogen：KLH-conjugated, synthetic peptide corresponding to amino acids 305-323 (KDSKDSLHANTANRRTTPV) of rat Stargazin at C-terminal. Species sequence homology: mouse
