MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Mitogen-activated protein (MAP) kinases are serine/threonine-specific protein kinases that respond to extracellular stimuli (mitogens, osmotic stress, heat shock and proinflammatory cytokines) and modulates cellular activities, such as proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-1ug/ml detects MKP1 in RIPA lysates from 3T3/A31 cells.
3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M4110-01 (0.3ug/ml).
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
MKP1 (~42kD)
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:F4209: Fibroblast Cell, 3T3/A31 lysate.
Add 2.5ul 2-mercaptoethanol/100ul of lysate.
Boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for Western Blot analysis.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human MKP1, Mr ~42kD. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa87-101, DERSAALDGAKRDGT-C of human MAP kinase phosphatase 1 (MKP1) conjugated to KLH, C-terminal cysteine added for conjugation purposes. Species Sequence Homology: rat
