MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: detects STAT5B (doublet of 90kD and 92kD) in human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure.
Immunoprecipitation: immunoprecipitates STAT5B from A431 carcinoma cells and a previous lot immunoprecipitated STAT5B from human Raji cells.
A RIPA lysate of human A431 cells (500ug protein) was incubated with 4ug of anti-STAT 5B Immunecomplexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B.
Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection
Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes the 90-92kD forms of human STAT5B protein. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：aa774-787 of human STAT5B (CGRPMDSQWIPHAQS)
