MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Nck is a highly conserved, oncogenic protein encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase-activating protein, phosphatidylinositol 3-kinase, and phospholipase C-gamma.
Nck is a common target for the action of different surface receptors.
Nck is widely expressed in various tissues and in cell lines from human, murine and rat origins.
Nck is phosphorylated on tyrosine, serine and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells, respectively.
Like other SH2-containing proteins, Nck is associated with tyrosine- autophosphorylated EGF or PDGF receptors via its SH2 domain.
Overexpression of Nck leads to transformation of NIH 3T3 cells.
The recombinant Nck precipitates endogenous WIP, a novel proline-rich protein that interacts with the Wiskott-Aldrich syndrome protein (WASP), from BJAB cell lysates.
Nck binds through its second SH3 domain to WIP, and Nck binds to WIP at a site (aa321-415) that differs from the WASP-binding site (aa416-488).
WIP has been shown to associate with the actin polymerization regulatory protein profilin and to induce actin polymerization and cytoskeletal reorganization in lymphoid cells.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes mouse Nck beta, Mr 47kD.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa175-190, EAPSFLSLRRGTALSN, from mouse Nck beta, conjugated to KLH. Species Sequence Homology: human
