MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Ductin is the major core component of gap junctions and recent structural data shows it to be a four alpha-helical bundle which fits particularly well into a low resolution model of the gap junction channel.
Ductin is also the main membrane component of the vacuolar H+-ATPase that is found in all eukaryotes and it seems likely that the gap junction channel first evolved as a housing for the rotating spindle of these proton pumps.
Because ductin protrudes little from the membrane, other proteins are required to bring cell surfaces close enough together to form gap junctions.
Such proteins may include connexins, a large family of proteins found in vertebrates.
Applications:Suitable for use in FLISA, Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunocytochemistry: 1:100-1:500Immunohistochemistry: 1:100-1:500 on frog and 1:100-1:2000 on chicken
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human ductin (the 16kD or c subunit of the V-ATPase). Has been specifically tested on flatworm (invertebrate), frog (vertebrate) and chick embryo tissue.
Isotype：IgG
Calc Applications Abbrev：FLISA IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Ductin (100% conserved in all species).
