MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Mutations of the BRAF gene implicated in human cancer.
Cancers arise owing to the accumulation of mutations in critical genes that alter normal programmes of cell proliferation, differentiation and death.
As the first stage of a systematic genome-wide screen for these genes, Davies et al.
(2002, UI:22082291) have prioritized for analysis signaling pathways in which at least one gene is mutated in human cancer.
The RAS (CG:176)-RAF (CG:93)-MEK (CG:1316)-ERK (CG:412)-MAP kinase pathway mediates cellular responses to growth signals.
Ras is mutated to an oncogenic form in about 15% of human cancer.
The three Raf genes code for cytoplasmic serine/threonine kinases that are regulated by binding RAS.
The authors reported BRAF somatic missense mutations in 66% of malignant melanomas and at lower frequency in a wide range of human cancers.
All mutations are within the kinase domain, with a single substitution (V599E) accounting for 80%.
Mutated BRAF proteins have elevated kinase activity and are transforming in NIH3T3 cells.
Furthermore, Ras function is not required for the growth of cancer cell lines with the V599E mutation.
As BRAF is a serine/threonine kinase that is commonly activated by somatic point mutation in human cancer, it may provide new therapeutic opportunities in malignant melanoma.
Applications:Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme
MAP Kinase Activation and MBP Phosphorylation: 4ug Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species Crossreactivity: rat and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa754-765, IQAGGYGAFPVH of human B-Raf, with an N-terminal cysteine added for conjugation to KLH.
