MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals.
Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities.
Rotaviruses are 70nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2.
The immunity acquired from exposure to rotavirus appears to be type specific following initial infection.
Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection.
The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
Applications:Suitable for use in Indirect Immunofluorescence and FLISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Grade：Highly Purified
Purity：Purified IgG fraction to a purity of >95%.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes ICPs and late structural (virion) antigens. >90% with human rotaviruses (reported). Uninfected Cell Reactivity: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence.
