MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Immunohistochemistry Protocol:1.
Dissect tissues.
Freeze on dry ice
2.
Cut on a cryostat ~10um slices
3.
Dry slides at RT for 30-90 minutes
4.
Fix with cold Acetone/Methanol (50/50) for 2 min then either process further or store at -20°C.
Alternative is fixation with 4% PFA and treatment with a trypsin solution (0.05% trypsin, incubation for 10 min.
at 37°C, followed by 3 washes with PBS)
5.
Let air dry
6.
Treat with PBS for 5 minutes
7.
Incubate in 50mM ammonium chloride for 30 minutes
8.
Treat with PBS for 5 minutes
9.
Incubate with Blocking Serum for 30-45 minutes
10.
Incubate with appropriately diluted L2075 for 90 minutes
11.
Wash 3 times for 5 minutes
12.
Incubate with fluorescent conjugated secondary anti-rabbit IgG for 30 minutes
13.
Wash 3 times for five minutes.
Mount.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes the human Lim homeobox containing transcription factor LHX2. No significant sequence homology with other proteins. Species Crossreactivity: rodent.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC
Calc Crossreactivity：Hu Rd
Immunogen：Synthetic peptide corresponding to aa239-254 af human LHX2/Lim2.
