MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Cofilin 2 is a small actin-binding protein that plays a pivotal role in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2.0ug/ml detects Cofilin 2 in RIPA lysates from rat L6 cell lysates.
Rat L6 lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7506-50 (1ug/ml).
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human Cofilin 2 at ~21kD. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa136-152, C-QVNGLDDIKDRSTLGEK, of human Cofilin 2 with N-terminal cysteine for KLH conjugation. Species Sequence Homology: mouse and chicken
