MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity.
AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kD) and non-catalytic beta and gamma subunits.
The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence.
AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation.
Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression.
Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detected AMP-activated Protein Kinase a1 in RIPA lysates from human non-stimulated A431 cells.
Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1475-01.
Proteins were visualized using an IgG (HRP) goat and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human AMPK a1, Mr 63kD. Species Crossreactivity: rat and mouse.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa376-392, C-ARHTLDELNPQKSKHQG-COOH of human AMP-activated Protein Kinase alpha1, conjugated to KLH (NP_006242).
