MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease.
SIRT5 is expressed in a variety of tissues.
Two different isoform have been described.
Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only.
SIRT5 has also been linked to many primate-specific functions.
SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlImmunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human SIRT5. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic linear peptide corresponding to human SIRT5 (KLH). Species sequence homology: chimpanzee and rhesus macaque (100%).
