MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion.
Antibody Anti-neurexin 3 alpha was made against a peptide immunogen corresponding to a stretch of aa’s in the extracellular domain of human neurexin 3 alpha.
Although the theoretical full length of Neurexin 1alpha is 180kD, the protein detected in human brain lysates may show a band around 180kD and additional bands around 40-55kD as a possible result of the posttranslational processing previously reported.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Requires antigen retrieval using heat treatment prior to staining paraffin sections.
Sodium citrate buffer, pH 6.0 is recommended
Optimal dilutions to be determined by the researcher.
Positive Control:PC12 cell lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity:Recognizes human Neurexin3. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:KLH-conjugated linear peptide corresponding to a stretch of amino acids in the extracellular domain of human Neurexin 3