MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Covalent modification of histones plays critical role in regulating chromatin structure and transcription.
JMJD8 is one member of the family of JmjC domain-containing histone demethylation (JHDM) enzymes.
These proteins remove specific methyl marks from histones.
Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate.
Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
While two isoforms of JMJD8 have been described, further study is still required to clarify the roles and mechanisms of JMJD8 among other JHDM protein family members.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa nuclear extract
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human JMJD8.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic linear peptide corresponding to human JMJD8 (KLH). Species sequence homology: Porcine, chimpanzee, rhesus, macaque, canine, equine (100%).
