MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
PP6 (PPP6, Protein Phosphatase 6) is a Ser/Thr protein phosphatase that is part of the type 2 PPP family that includes PP2A, PP4, and PP6.
All of these phosphatases are sensitive to low doses of Okadaic acid.
PP6 is conserved among eukaryotes and the function of PP6 is relatively unknown, but the Saccharomyces cerevisiae homolog of Sit4 has been implicated in the cell regulation of G1 to S progression (Stefansson, 2006).
Knockdown of PP6 in mammals has been related to a very high rate of apoptosis.
Based on this and other information, PP6 is believed to function in cell cycle regulation.
PP6C encodes the catalytic subunit of the protein phosphatase.
PP6C binds to PP6R1, PP6R2, and PP6R3 through the SAPS domain of PP6R (Stefansson, 2006).
This complex is known to bind to and protect IKBE from degradation in response to TNF alpha treatment (Stefansson, 2006).
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry (paraffin): 1:100Immunoprecipitation: 10-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kD. Species Crossreactivity: Human, Mouse, Rat, Bovine.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of PP6C.
