MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Cytosolic oxidase-associated protein, a component of the nicotinamide adenine dinucleotide phosphate-oxidase, which mediates down-regulation of NADPH oxidase through interactions with its SH3 domain.
Associated with p67-phox but is absent in patients with chronic granulomatous disease who lack p67-phox.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml detects p40 phox in RIPA lysates of HL-60 cells.
HL-60 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P1001-20C (1ug/ml).
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human p40 phox, Mr ~40kD. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：C-terminal 6His-tagged fusion protein corresponding to full length human p40 phox
