MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
The LIM motif defines a zinc-binding domain that is found in a variety of transcriptional regulators and proto-oncogenes products.
LIM domain mediates protein-protein interactions and, with LIM-homeodomain proteins, act as negative regulators of the transcriptional activation function of the protein.
This transcription factor is required for embryonic survival and for the determination of many cell types.
Applications:Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Immunoprecipitation.
Western Blot: 1:3000-1:6000 Immunocytochemistry: 1:500 on P19 cell line Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：frog
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes frog Lim-1. Does not crossreact with Lim-5 (the closest homolog of Lim-1 in frog) on tissue sections but does crossreact with Lim-5 in Western Blot and Immunoprecipitation. Species Crossreactivity: human, rat, mouse and fish. Also reacts with Lim-1 from whole-mount fish embryo.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Fr Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-terminal portion of frog Lim-1.
