MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Immunoblot, FLISA, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunoblot: 1:2500-1:5000 FLISA: 1:2000-1:4000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes most mammalian laminins and has negligible crossreactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific crossreaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible. Typically less than 1% crossreactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
