MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Detects a band of the proper molecular weight, 30kD, in lysates from E.
coli expressing GFP, but not in E.
coli that do not express or in lysates prepared from E.
coli containing the GFP-expressing plasmid but not induced, detect GFP in lysates from COS or primary neurons or fibroblast cells transfected with plasmids directing expression GFP or a tau-GFP fusion protein.
Did not detect any signal when 50ug of protein from a rat brain lysate (negative control) was fractionated on the SDS-PAGE.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Immunocytochemistry: Cells were fixed using 4% formaldehyde in PBS, pH 7.4 for 20 minutes at RT.
The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the animal in which the secondary antibody was raised) in PBS for 20 minutes at RT.
Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4°C, overnight.
Has also been used successfully with the avidin/biotin/peroxidase detection system.
Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine/ 0.02% hydrogen peroxide solution on ice for 8-10 minutes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：chicken
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes Green Fluorescent Protein (GFP). Has been used to detect GFP in primary neurons and zebrafish fibroblasts. Reactive with GFP from both native and recombinant sources. Species Crossreactivity: vertebrates
Isotype：IgY
Calc Applications Abbrev：IC IP WB
Immunogen：Recombinant protein corresponding to Green Fluorescent Protein (GFP) containing a 6-His tag.
