MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Western blot: 1:10 using ECL Immunoprecipitation: 1:10 following the protocol of Raymond et al, Nature (1993) 361:637–641.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Affinity Purified
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity:Recognizes glycine receptor alpha 1. Shows crossreactivity to glycine receptor alpha 2. subunits Used successfully in Western blot and immunoprecipitation assays with cell extracts containing recombinant human glycine receptors and with homogenates of mouse spinal cord. By Western blot recognizes a protein with a MW of ~48kD. Species Reactivity: Human and mouse
Isotype:IgG
Calc Applications Abbrev:IP WB
Calc Crossreactivity:Hu Mo
Immunogen:Peptide from the amino terminus of the human alpha 1 glycine receptor subunit conjugated to KLH.