MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Dot and Slot blot, FLISA, Immunohistochemistry, and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:*Dot and Slot blot: 1:100-1:500 FLISA: 1:1000-1:4000 *Immunohistochemistry (Frozen): 1:10-1:40 Immunocytochemistry: 1:10-1:20 Optimal dilutions to be determined by the researcher.
*Note: Harsh chemicals or procedures involving heat may denature collagen epitopes.
We recommend avoiding such actions when using this antibody.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes the conformational determinants on human type IV collagen. Less than 10% crossreactivity with collagen types I, II, III, V and VI. May show reactivity to type IV collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin). Species Crossreactivity: bovine and mouse
Isotype：IgG
Calc Applications Abbrev：DB FLISA IC IHC
Calc Crossreactivity：Bo Hu Mo
Immunogen：Human and bovine placental collagen type IV
Shelf Life：1year
Swiss Prot Number：P02462
EU Commodity Code：30021010
商品名：Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, DKFZp686I14213, FLJ22259, Goodpasture antigen) (MaxLight 490) 100ul
