MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Type I collagen is the most common of the collagens in vertebrates.
It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body.
Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations.
The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc.
Type I collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions.
Applications:Suitable for use in Dot and Slot Blot, FLISA, Indirect Immunohistochemistry and Indirect Immunocytochemistry.
Not recommended for paraffin tissue sections.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity:Recognizes human type I collagen. Crossreactivity (ELISA): <10% collagen types II, III, IV, V and VI. May show reactivity to type I collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Isotype:IgG
Calc Applications Abbrev:DB FLISA IC IHC
Calc Crossreactivity:Hu
Immunogen:Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.