MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content.
It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells.
Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth.
Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging.
It strengthens blood vessels and plays a role in tissue development.
It is present in the cornea and lens of the eye in crystalline form.
Collagen occurs in many places throughout the body.
There are 12 types of collagen described in literature.
Collagen Type 1: This is the most abundant collagen of the human body.
It is present in scar tissue, the end product when tissue heals by repair.
It is found in tendons, the endomysium of myofibrils and the organic part of bone.
Collagen Type 4: It is present in basal lamina and the eye lens.
Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.
Applications:Suitable for use in Immunohistochemistry, RIA, Western Bot and FLISA.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Using frozen mouse skin and liver tissuesOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes mouse Collagen Type 1. <0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagen Type I. 1% reactivity with mouse Collagen Type III.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC RIA WB
Calc Crossreactivity：Mo
Immunogen：Collagen Type 1 extracted and purified from mouse skin.
