MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors.
Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions.
The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties.
Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C.
Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8.
Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
Multiple transcript variants encoding different isoforms have been found for this gene.
Metabotropic glutamate receptor 7 (mGLUR7), also called GRM7 (G protein-coupled metabotropic glutamate receptor 7) is a 102kD member of the GRM family of 7-transmembrane G protein-coupled receptors.
MGLUR7 is concentrated in the hippocampus and the amygdala and has a putative role in anxiety and spatial working memory.
GRM7 has been reported to be expressed in active zones of presynaptic axon terminals in various regions of the brain, eye, and spinal cord.
Caution: GLUR7 refers to Ionotropic Glutamate Receptor 7, not to Metabotropic Glutamate Receptor 7 (mGLUR7
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by researcher.
Storage and Stability: Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human mGluR7 at Mr 97kD. Species crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa899-912, C-NSPAAKKKYVSYNN, of human mGluR7, conjugated to KLH.
