MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Neuronal precursor cell-expressed developmentally downregulated 4 (Nedd4) is an ~120kD E3 ubiquitin ligase.
Nedd4 is involved in regulation of ubitiquin-targeted degradation of proteins.
Nedd4 interacts with many different E2 enzymes, including human Ubc4, UbcH5B, UbcH5C, UbcH6 and UbcH7, suggesting it plays a role in multiple E2 ubiquitination pathways.
Nedd4 contains a C2 domain and several WW domains, which recognize membrane surfaces and Pro-Tyr motifs, respectively.
Nedd4 polyubiquitinates the epithelial sodium channel, and mutations that disrupt association between Nedd4 and the channel lead to Liddle’s syndrome, a hyperaldosteronism-like, hypertension disease.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:10,000 detects Nedd4, WW2 domain on 10ug of mouse kidney cytosol.
Mouse kidney cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with N0577-15.
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
Immunohistochemistry: Reported to detect Nedd4 in mouse kidney, lung and distal colon sections
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Positive Control:Kidney cytoplasmic extracts
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes rat Nedd4, Mr ~115kD. Degradation products were also detected at Mr 55 and 60kD. Species Crossreactivity: mouse and human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：GST fusion protein corresponding to aa395-462 of the WW2 domain of rat Nedd4 (NM_006154).
