84-3372-25　［取扱停止］Fz5 (FZD5, Frizzled Homolog 5 (Drosophila), C2orf31, Chromosome 2 Open Reading Frame 31, DKFZp434E2135, DKFZP434E2135, Frizzled-5, Frizzled 5 precursor, Frizzled-5 precursor, Fz-5, FzE5, hFz5, Hfz5, HFZ5, MGC129692, Uncharacterized Protein C2orf31) (MaxLight 490) 100ul　F9900-ML490

特徴

• MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• The Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis.
• The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), beta- and gamma- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors.
• Frizzled 5 is believed to function as the receptor for the Wnt5a ligand.
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:1000.
• Lysates from mouse liver, C2C12, Jurkat and HUVEC cells were resolved by electrophoresis, transferred to PVDF and probed with F9900
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Positive Control:Mouse liver, C2C12, Jurkat and HUVEC cells
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™490 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.