MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Calreticulin was originally identified as a Ca2+ binding protein in the muscle sarcoplasmic reticulum.
Since its original discovery, it has been found in high abundance in non-muscle tissue.
Calreticulin has the ability to bind Ca2+ with high capacity but low affinity.
The exact function of calreticulin is not yet known, however recent reports suggest that it may play a role in nuclear hormone receptor gene transcription and may also act as a chaperone protein.
Calreticulin is 90% conserved among human, mouse, rat, rabbit and Aplysia.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Detects calreticulin in tissue sections and cells.
Optimal diltions to be determined by the researcher.
Included Positive Antigen Control:F4209: Fibroblast Cell, 3T3/A31 lysate.
Add 2.5ul 2-mercaptoethanol/100ul of lysate.
Boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for Western Blot analysis.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human calreticulin. Does not crossreact with other endoplasmic reticulum proteins. Species Crossreacitivity: mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt Xe
Immunogen：Synthetic peptide corresponding aa412-417, QAKDEL, at the C-terminus of human calreticulin, conjugated to KLH.
