84-3372-06　［取扱停止］Platelet Derived Growth Factor Receptor Type B (PDGFRb) (MaxLight 490) 100ul　P4258-23-ML490

特徴

• MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• Two types of PDGF receptors have been identified: PDGF receptor A (PDGFRa) and PDGF receptor B (PDGFRb).
• The PDGFRa, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGFRb, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity.
• Both receptors are transmembranous, ligand-activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains.
• The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment.
• Applications:Suitable for use in FLISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Positive Antigen Control Included:F4209: 3T3/A31 cell lysate.
• Use 20ug/lane for minigels
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™490 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.