MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Protein phosphatase 5 (PP5) is a novel human protein serine/threonine phosphatase of molecular weight of 58kD.
It is made up of a C-terminal phosphatase catalytic domain and an N-terminal domain, which has four repeats of 34 amino acids, three of which are tandemly arranged.
The phosphatase domain contains all of the invariant motifs of the PP1/PP2A/PP2B gene family.
However, it is not closely related to any other known member of this family of phosphatases.
Thus it makes up a new subfamily of phosphatases.
PP5 expressed in bacteria has been shown to dephosphorylate serine residues in proteins and is more sensitive than PP1 to the tumor promoter okadaic acid.
PP5 appears to be localized to the nucleus where it is thought to play a roll in the regulation of RNA biogenesis and mitosis as part of a signaling pathway regulating cell cycle progression.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation (paraffin): 1:100Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate, A549 and MCF-7 cell lysates.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human protein phosphatase 5. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide derived from the human protein phosphatase 5. Cellular Localization: Nucleus. Cytoplasm. Epitope: C-terminus.
