MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Steroidogenic factor (SF-1), is an orphan nuclear receptor that regulates the enzymes that produce steroids and is required for adrenal and gonadal development.
SF-1 is important in the regulation of the pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
SF-1 is also expressed at high levels in the embryonic diencephelon that gives rise to the endocrine hypothalamus.
Therefore, SF-1 plays an important role in the development and function of multiple endocrine organs that are essential for reproduction.
SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family.
SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: detects SF-1 in RIPA lysates from Y1 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes mouse SF-1, Mr 53kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：DNA binding domain of SF-1 from mouse, produced in E. coli as a GST fusion protein and partially purified via GST-agarose column chromatography.
