MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Recommended Dilution:Immunoblot Analysis: 0.5-2.0ug/ml of this lot detected phospho-NR2B in a rat brain microsomal protein preparation
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes phospho-NR2B, Mr 180kD. A non-specific protein was also detected, Mr 46kD, which can serve as a loading control. Species Crossreactivity: Human and rat. Species sequence homology: human, rat, and mouse 100%
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa1297-1309 (CKLRRQH( pS)YDTFVD) of human phospho-NR2B with an N-terminal cysteine added for conjugation (KLH).
