MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunoblot: 0.5-2ug/ml of this lot detected a Myc epitope tagged protein in a transfected Cos-1 cell lysate.
1ug/ml of a previous lot detected endogenous Myc in HeLa nuclear extract.
A lysate of transfected Cos-1 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myc Tag (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Myc epitope tagged protein (~66kD)Immunocytochemistry: Reported to detect Myc-tagged proteins in transfected cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN)
