84-3370-16 [取扱停止]Kip1, p27, phosphorylated (Thr187) (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) (MaxLight 405) 100ul K1849-10-ML405
特徴
MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: detected phosphorylated recombinant p27 Kip1-GST fusion protein.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity:Recognizes phosphorylated site in native human Kip1, Mr 27kD, and recombinant p27 Kip1-GST, Mr55kD. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:KLH-conjugated, synthetic peptide (C-PNAGSVEQ[pT]PKK) corresponding to amino acids 179-190 of human Kip1. Species sequence homology: feline
Shelf Life:1year
Swiss Prot Number:P46527
EU Commodity Code:30021010
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