MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
HuR (Hu-antigen R, ELAV1) is an RNA binding protein that contains 3 RNA-binding domains.
It is known to bind AU-rich elements in mRNA.
It regulates gene expression by destabilizing their mRNA.
It is known to be upregulated in certain cancers such as breast and gastric cancers.
Some of its targets include cFos, IL-3, and BRCA1 mRNAs.
HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types.
It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Reported to detect HuR in rabbit placental tissue
Immunocytochemistry: 1:500.
Detects HuR in HeLa and NIH/3T3 cell lysates
Western Blot: 1ug/ml.
Detected HuR in RIPA lysates from 3T3/A31 cells.
3T3 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with H7953-34.
Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system
Immunoprecipitation: 10ug immunoprecipitated HuR in 3T3/A31 RIPA lysates
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included):F4209: Fibroblast Cell, 3T3/A31: Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human HuR, Mr ~36kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa1-13, MSNGYEDHMAEDC, of human HuR, conjugated to KLH (NP_001410). Species Sequence Homology: monkey (100%)
