84-3369-82 [取扱停止]SOS1 (Son of Sevenless Homolog 1, SOS-1) (MaxLight 405) 100ul S5360-18-ML405
特徴
MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control Included:F4209: Fibroblast Cell, 3T3/A31.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity:Recognizes mouse SOS1, Mr 175kD. Species Crossreactivity: human.
Isotype:IgG
Calc Applications Abbrev:IP WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide corresponding to aa1243-1258, KKSDHGNAFFPNSPSP of murine Son of Sevenless Homolog 1 Protein (KLH).
Shelf Life:1year
Swiss Prot Number:Q62245, Q07889
EU Commodity Code:30021010
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