MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
This gene is intronless and encodes a member of the histone H2B family.
Transcripts from this gene contain a palindromic termination element.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: detected Histone H2B in acid extract of HeLa cells treated with either sodium butyrate or colcemid.
Optimal dilutions to be determined by the researcher.
Positive Control:Acid extract from either sodium butyrate or colcemid treated HeLa cells.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human Histone H2B. It targets an epitope within 9 amino acids from the C-terminus region. Species Crossreactivity: chicken
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ch Hu
Immunogen：KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminus of human Histone H2B type 3-B. Species sequence homology: yeast and xenopus
