MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
The CD44 molecule belongs to a family of cellular adhesion molecule found on a wide range of normal and malignant cells in epithelial, mesothelial and haematopoietic tissues.
CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (HCAM) molecule.
The additional 10 exons (V1 to V10) are only expressed by alternative splicing of the nuclear RNA.
The expression of specific cell adhesion molecule CD44 splice variants has been shown to be associated with metastasis and poor prognosis in certain human malignancies, such as breast cancer.
Applications:Suitable for use in RIA, Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Functional Blocking.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human CD44v6 (~200-250kD). Species Crossreactivity: rabbit, porcine, mouse, canine and bovine
Isotype：IgG
Calc Applications Abbrev：FC FLISA IF IHC IP RIA WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rb
Immunogen：Synthetic peptide corresponding to human CD44v6.
