MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
A disintegrin and metalloproteinase domain-containing protein 9 (ADAM 9) belongs to a family of transmembrane, membrane-anchored, disintegrin-containing proteins that are involved in several biological functions such as protein ectodomain shedding, cell fusion, and cell adhesion.
ADAMs contain several characteristic domains, two of which, the protease and disintegrin domains, are noted for their homology to snake venom metalloprotease.
The exact function of ADAM 9 is yet unknown, however it is widely distributed, is found within keratinocytes, and is ideally placed on the cell membrane, which some studies have suggested supports a role in cell migration and motility.
Alternative splicing results in two isoforms; isoform 1 is found within the cell membrane and is considered a single-pass type 1 membrane protein, while isoform 2 is secreted.
Mutations in ADAM 9 expression are causal to cone-rod dystrophy type 9 or CORD9 which is characterized by visible retinal pigment deposits and loss of cone photoreceptors leading and rod degeneration, ultimately resulting in severe loss of vision.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.5ug/ml.
Evaluated by Western Blot in human chondrocyte cell lysate
Optimal dilutions to be determined by the researcher.
Positive Control:Human chondrocyte cell lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human ADAM 9, Disintegrin Domain (A Disintigrin And Metalloproteinase 8, MDC9, Meltrin-g). Predicted to react with primate and chimpanzee based on 100% sequence homology. Species Crossreactivity: Mouse and rat (90% sequence homology).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated linear peptide corresponding to the disintegrin domain of human ADAM 9.
