Dopaminergic signals are thought to modulate corticostriatal synapses onto medium spiny projection neurons, and this plasticity is, in turn, thought to alter patterns of activity during learning.
Subversion of these processes in clinical and rodent models is hypothesized to contribute importantly to addiction to psychostimulants, seizures, and movement disorders in Huntington’s (HD) and Parkinson’s disease (PD).
Dopamine receptor D1, also known as DRD1, is the most abundant dopamine receptor (of the five types D1-D5) in the central nervous system and is critically involved in these processes and the modulation of D2 receptor mediated events.
Coupled with G-protein, the D1a receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases including the key molecular switch, DARPP-32.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100Immunohistochemistry: 1:300Optimal dilutions to be determined by the researcher.
Control:Rat testis tissue lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes rat Drd1. Species Crossreactivity: bovine.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Rt
Immunogen：Linear peptide of rat dopamine Drd1 at and around the cytoplasmic domain, conjugated to KLH.
