S-Adenosyl-I-methionine-dependent protein arginine N-methyltransferases (PRMTs) methylate arginine residues within a variety of proteins, particularly histones.
At least four distinct mammalian family members have now been described, including PRMT1, PRMT3, CARM1/PRMT4 and JBP1/PRMT5
Applications:Suitable for use in Western Blotting, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100-1:500.
Reacts with a band of approximately 57kD on whole HL60 cell lysate.
Additional bands may be present depending on sample used
ELISA (direct): 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified immunoglobulin
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human PRMT3 (Protein Arginine N-Methytransferase 3).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide from N-terminus of human PRMT3.
