Applications:Suitable for use in Western Blotting.
Other applications not tested.
Recommended Dilution:Western blotting: 1:200 using ECL on rat brain membranes
Optimal dilutions to be determined by the researcher.
Control Antigen Included: 40ug, lyophilized.
For negative control, preincubate 1ug of purified peptide with 1ug of antibody for one hour at RT.
Optimal concentrations must be determined by the end user.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity:Recognizes P2X6 Receptor. The epitope specific for the P2X6 receptor is not present in any other known proteins. Species Crossreactivity: Rat. The immunogen sequence is conserved in mouse (15/15) and human (14/17).
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Rt
Immunogen:Highly purified peptide corresponding to residues 363-379 of rat P2X6 (UniProt #P51579).