TIMP-1and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity.
TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B).
TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 5-10ug/ml.
Recognizes a band at ~28-30kD in reduced samples
Immunohistochemistry: 1ug/sectionELISA: 5ug/mlOptimal dilution to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human TIMP (Tissue Inhibitor of Metalloproteinase). Recognizes native and denatured TIMP and TIMP in and out of enzyme/inhibitor complex. Does not crossreact with other human proteins tested. Not known to inhibit biological activity. Species Crossreactivity: rabbit, sheep, bovine, rat, equine and ferret
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Eq Hu Rb Rt Sh
Immunogen：Full length purified human fibroblast TIMP (Tissue Inhibitor of Metalloproteinase).
