The p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains.
However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions.
p73 is expressed in either a full-length form (a) (MW ~80kD) or a shorter (b) (MW ~70kD) mRNA variant, with exon 13 spliced out and is especially designed for a sensitive detection of both p73a and p73b in Immunoprecipitation and Western Blot assays.
Transient overexpression of p73 in cells can induce apoptosis and p21, a cellular p53 target gene primarily responsible for p53-dependent cell cycle arrest.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1ug/ml of the antibody will detect p73 in 30ug of total cell lysate from overexpressing cell lines.
Higher levels of antibody may be necessary when using native tissue sources
Immunohistochemistry: It may be necessary to incorporate a signal amplification technique in conjunction with standard procedures
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：> 95% (SDS-PAGE). Purified by affinity chromatography and 0.22um filtered.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes amino acids 450-636 of human p73 protein. This region is common to both p73 alpha and p73 beta isoforms, hence both isoforms of the p73 protein will be detected.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：GST fusion protein that contains aa450-636 of the p73 protein.
