84-3365-67　Nkx 5.1 (NK-2 Family Homeodomain Transcription Factor) (PE) 100ul　N2851-PE

特徴

• Applications:Suitable for use in FLISA, Immunohistochemistry, Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Immunohistochemistry Protocol:1.
• Dissect tissues and freeze on dry ice 2.
• Cut on a cryostat -10um slices 3.
• Dry slides at RT for 30-90 minutes4.
• Fix with cold Acetone/Methanol (50/50) 2 minutes - then either process further or store at -20°C .
• Alternative is fixation with 4% PFA and treatment with a trypsin solution.
• (0.05% trypsin, incubation 10 minutes at 37°C, followed by 3 washes with PBS5.
• Let air dry 6.
• PBS 5 minutes 7.
• Incubate in 50mM ammonium chloride 30 minutes 8.
• PBS 5 minutes 9.
• Blocking serum 30-45 minutes 10.
• N2851 90 minutes - dilution 1:200 - 1:1000 11.
• Wash 3 times for 5 minutes 12.
• Secondary fluorescent conjug.
• antibody 30 minutes 13.
• Wash 3 times for 5 minutes and mount
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.