84-3365-64　Matrix Metalloproteinase 2 (MMP-2, Collagenase IV, Gelatinase A) (PE) 100ul　M2420-66-PE

特徴

• MMP-2 is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen.
• The low protein levels produced in cell culture (pg/ml) are often below the threshold of detection by standard Western Blotting.
• The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I.
• et al.
• J.
• Biol.
• Chem.
• 267, 4583–4591.
• 1992) or immunoprecipitation.
• This can prevent appearance of a spurious band of antibody binding in the vicinity of 68kD displayed by some concentrated media.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions: Optimal dilution determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.