Glucose is fundamental to the metabolism of mammalian cells.
Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling.
GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast.
GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 5ug/ml, adipose and muscle tissueImmunohistochemistry: 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography on a Sepharose 4B CNBr linked peptide column.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human glucose transporter 1 from muscle and adipose tissue. Does not crossreact with GLUT-4. The MW of the glucose transporter precipitated by the serum is ~46kD.Species Crossreactivity: rat and mouse
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to 12aa, LFHPLGADSQV, of the C-terminus of human GLUT-1, conjugated to KLH.
