Applications:Suitable for use in Immunohistochemistry, RIA and ELISA.
Not suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: 1:80.
Frozen mouse skin and liver tissuesImmunohistochemistry: Acetone or methyl-carnoy fixed, paraffin-embedded tissueRIA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity:Recognizes Mouse Collagen Type IV: 100%, Mouse Collagen Types I, II, III: < 0.1%, Mouse Fibronectin: < 0.1%, Mouse Laminin: < 0.01%, Human Collagen Types IV, V: < 0.1%.
Isotype:IgG
Calc Applications Abbrev:E IHC RIA
Calc Crossreactivity:Mo
Immunogen:Collagen type IV extracted and purified from mouse tumor tissues