Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells.
Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes.
These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
Applications:Suitable for use in Western Blot, Dot Blot, Chromatin Immunoprecipitation and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Histone H3 (Mr 17kD) acetylated at position Lys 9. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：DB FC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-12, ARTKQTAR(AcK)STG-C, where (AcK) denotes acetyl-lysine of human Histone H3, conjugated to KLH (NP_003520). Peptide sequence is identical in a wide range of animal and plant species.
